Institute for Advanced Biosciences, Keio UniversityInstitute for Advanced Biosciences, Keio University

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Mitsuhiro Itaya

Mitsuhiro Itaya
Institute for Advanced Biosciences
Keio University

mita at
Institute for Advanced Biosciences
403-1 Daihoji, Tsuruoka, Yamagata
997-0017 Japan
Tel: Fax: +81-235-29-0529

Degree / Specialization:
Ph.D., University of Tokyo
Molecular Biology, Biochemistry, etc.

Short Biography:
Dr. Mitsuhiro Itaya received his Ph.D. in Biophysics and Biochemistry from University of Tokyo (1983). He spent three years (1983-1986) as a post-doctoral fellow at the National Institutes of Health, Bethesda, MD, USA in Molecular Genetics. Since 1986 he had been a staff researcher in Mitsubishi Kagaku Institute of Life Sciences, Machida, Tokyo, before joining the faculty at Keio in 2006. He shares his time between research and teaching at both undergraduate and graduate levels.

Research Activities & Projects:
Genome designing, Genome engineering, Bacterial genetics. My research is focused on creation of biological systems that are subject to various analyses for integrative understanding of what life is. My laboratory has developed methods for handling of giant DNA, that is, the whole genomes that originate from various viruses, organella, and bacteria. The recombinant genomes thus being constructed should be novel and unprecedented subjects that may lead to the experimental basis for engineered life.

genome designing, genome engineering, genome structure, genomics

Itaya, M., Sakaya, N., Matsunaga, S., Fujita, K., and Kaneko, S. Conjugational transfer kinetics for Bacillus subtilis in liquid culture. Biosci. Biotechnol. Biochem. 70, 740-742 (2006).

Itaya, M., Tsuge, K. Koizumi, M., and Fujita, K. Combining two genomes in one Cell: Stable cloning of the Synechosystis PCC6803 genome in the Bacillus subtilis 168 genome.Proc. Natl. Acad. Sci., USA, 102, 15971-15976 (2005).

Kaneko, S, Akioka, M., and Itaya, M. DNA shuttling between plasmid vectors and a genome vector: Systematic conversion and preservation of DNA libraries using the Bacillus subtilis genome (BGM) vector. J. Mol. Biol. 349: 1036-1044 (2005).

Ohashi, Y., A. Yamashiro, T. Washio, N. Ishii, H. Ohshima, T. Michishita, M. Tomita, and M. Itaya, In silico diagnosis of inherently inhibited gene expression focusing on initial codon combinations. Gene 347: 11-19 (2005).

Ohtani, N., Saito, N., Tomita, M., Itaya, M., and Itoh, A. The SCO2299 gene fromStreptomyces coelicolor A3(2) encodes a bifunctional enzyme consisting of an RNase H domain and an acid phosphatase domain. FEBS Journal 272, 2828-2837 (2005).

Qiu D., K. Fujita, Y. Sakuma, T. Tanaka, Y. Ohashi, Ohshima, H., Tomita, M., and Itaya, M. Comparative analysis of the structure of complete physical maps of four Bacillus subtilis (natto) genome. Appl. Environ. Microbiol. 70: 6247-6256 (2004).

Tomita, S., Tsuge, K., Kikuchi, Y., and Itaya, M. Targeted isolation of a designated region of the Bacillus subtilis 168 genome by Recombinational transfer. Appl. Environ. Microbiol. 70: 2508-2513. (2004).

Ohtani, N., Yanagawa, H., Tomita, M., and Itaya, M. Cleavage of double stranded RNA by RNase HII from a Thermoacidophilic Archaeon, Sulfolobus tokodaii 7. Nucleic Acids Res. 32: 5809-5819 (2004).

Ohashi, Y., H. Ohshima, K. Tsuge, and M. Itaya Far different levels of gene expression provided by an oriented cloning system in Bacillus subtilisand Escherichia coli. FEMS Microbiol. Lett. 221:125-130 (2003).

Tsuge, K.�AMatsui, K., and Itaya, M. One step assembly of multiple DNA fragments with designed order and orientation in Bacillus subtilis plasmid. Nucleic Acids Res. 31: No. 21, e-133 (2003).

Kaneko, S., Tsuge, K., Takeuchi, T., and Itaya, M. Conversion of Submegasized DNA to Desired Structures Using A Novel Bacillus subtilis Genome Vector. Nucleic Acids Res.31: No. 18, e112 (2003).

Tsuge, K. and Itaya, M. Recombinational transfer of 100-kb genomic DNA to plasmid inBacillus subtilis 168. J. Bacteriol., 183, 5453-5456 (2001).

Itaya, M. Genetic transfer of large DNA inserts to designated loci of the Bacillus subtilis168 genome. J. Bacteriol. 181, 1045-1048 (1999).

Itaya, M., Omori, A., Kanaya, S., Crouch, RJ., Tanaka, T., and Kondo, K. Isolation of RNase H genes that are essential for growth of Bacillus subtilis 168. J. Bacteriol. 181, 2118-2123 (1999).

Kunst, F., Ogasawara, N., ..Itaya, M..(146 authors).. and Dunchin, A. The complete genome sequence of the Gram-positive bacterium Bacillus subtilis. Nature, 390, 249-256 (1997).

Itaya, M. and Tanaka.T. Experimental surgery to create subgenomes of Bacillus subtilis168. Proc. Natl. Acad. Sci., USA. 94, 5378-5382 (1997).

Itaya, M. Physical map of the Bacillus subtilis 166 genome: Evidence for the inversion of an approximately 1900 kb continuous DNA segment, the translocation of an approximately 100 kb segment, and a duplication of a 5 kb segment. Microbiology 143, 3723-3732 (1997)

Itaya, M. Toward a bacterial genome technology: Integration of the Escherichia coliprophage lambda genome into the Bacillus subtilis 168 chromosome. Mol. Gen. Genet.,248, 9-16 (1995).

Toda, T. and Itaya, M. I-CeuI recognition sites in the rrn operons of the Bacillus subtilis168 chromosome: inherent landmarks for genome analysis. Microbiology 141, 1937-1945 (1995).

Itaya, M. An estimation of minimal genome size required for life. FEBS Lett. 362, 257-260 (1995).

Itaya, M. Stability and asymmetric replication of the Bacillus subtilis 168 chromosome. J. Bacteriol. 175, 741-749 (1993)

Kanaya, S. and Itaya, M. Expression, purification, and characterization of a recombinant ribonuclease H from Thermus hermophilus HB8. J. Biol. Chem. 267, 10184-10192 (1992)

Itaya, M. and Tanaka, T. Complete physical map of the Bacillus subtilis 168 chromosome constructed by a gene-directed mutagenesis method. J. Mol. Biol. 220, 631-648 (1991)

Itaya, M. and Kondo, K. Molecular cloning of a ribonuclease H (RNase HI) gene from an extreme thermophile Thermusthermophilus HB8: a thermostable RNase H can functionally replace the Esherichia coli enzyme in vivo. Nucleic Acids Res. 19, 4443-4449 (1991)

Itaya, M. and Crouch, RJ. A combination of RNase H (rnh) and recBCD or sbcB mutations in E. coli K-12 adversely affects growth. Mol. Gen. Genet., 227, 424-432 (1991)

Itaya, M. Isolation and characterization of a second ribonuclease H (RNase HII) ofEsherichia coli K12 encoded by the rnhB gene. Proc. Natl. Acad. Sci., USA. 87,8587-8591 (1990)